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验证数据展示

  • WB analysis using 16650-1-AP

    WB analysis using 16650-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16650-1-AP (LDB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 16650-1-AP

    WB analysis of HEK-293 using 16650-1-AP

    WB result of LDB1 antibody (16650-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDB1 transfected HEK-293 cells.

  • IP experiment of MCF-7 using 16650-1-AP

    IP experiment of MCF-7 using 16650-1-AP

    IP result of anti-LDB1 (IP:16650-1-AP, 4ug; Detection:16650-1-AP 1:1500) with MCF-7 cells lysate 1000 ug.

LDB1 Polyclonal antibody

LDB1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 16650-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

CLIM 2, CLIM2, hLdb1, LDB 1, LDB1, LIM domain binding 1, LIM domain binding protein 1, NLI, Nuclear LIM interactor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy LDB1 Ready-To-Use IHC Kit. LDB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16650-1-AP targets LDB1 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 LDB1 fusion protein Ag10082 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM domain binding 1
别名 CLIM 2, CLIM2, hLdb1, LDB 1, LDB1, LIM domain binding 1, LIM domain binding protein 1, NLI, Nuclear LIM interactor
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC000482
基因名称 LDB1
Gene ID (NCBI) 8861
RRIDAB_2137010
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86U70
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIM domain-binding protein 1 (LDB1), also known as Carboxyl-terminal LIM domain-binding protein 2 (CLIM2), binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. LDB1 may regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. It plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. LDB1 acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. It also acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state. LDB1 strongly interacts with the LIM2 domain of LMX1A to form homodimer. LDB1 protein is expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Due to alternative splicing events, three isoforms exist for LDB1. This antibody reacts with isoform 1.

实验方案

Product Specific Protocols
WB protocol for LDB1 antibody 16650-1-APDownload protocol
IP protocol for LDB1 antibody 16650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols