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验证数据展示

  • WB analysis using 16650-1-AP

    WB analysis using 16650-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16650-1-AP (LDB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 16650-1-AP

    WB analysis of HEK-293 using 16650-1-AP

    WB result of LDB1 antibody (16650-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDB1 transfected HEK-293 cells.

  • IP experiment of MCF-7 using 16650-1-AP

    IP experiment of MCF-7 using 16650-1-AP

    IP result of anti-LDB1 (IP:16650-1-AP, 4ug; Detection:16650-1-AP 1:1500) with MCF-7 cells lysate 1000 ug.

LDB1 Polyclonal antibody

LDB1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 16650-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

CLIM 2, CLIM2, hLdb1, LDB 1, LDB1, LIM domain binding 1, LIM domain binding protein 1, NLI, Nuclear LIM interactor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy LDB1 Ready-To-Use IHC Kit. LDB1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16650-1-AP targets LDB1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen LDB1 fusion protein Ag10082 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LIM domain binding 1
Synonyms CLIM 2, CLIM2, hLdb1, LDB 1, LDB1, LIM domain binding 1, LIM domain binding protein 1, NLI, Nuclear LIM interactor
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC000482
Gene Symbol LDB1
Gene ID (NCBI) 8861
RRIDAB_2137010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86U70
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIM domain-binding protein 1 (LDB1), also known as Carboxyl-terminal LIM domain-binding protein 2 (CLIM2), binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. LDB1 may regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. It plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. LDB1 acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. It also acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state. LDB1 strongly interacts with the LIM2 domain of LMX1A to form homodimer. LDB1 protein is expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Due to alternative splicing events, three isoforms exist for LDB1. This antibody reacts with isoform 1.

实验方案

Product Specific Protocols
WB protocol for LDB1 antibody 16650-1-APDownload protocol
IP protocol for LDB1 antibody 16650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols