• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

LDHA Monoclonal antibody, PBS Only

LDHA Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E2G6

Cat No : 66287-1-PBS

Print datasheet

Synonyms

lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, PIG19

验证数据展示

  • WB analysis using 66287-1-Ig (same clone as 66287-1-PBS)

    WB analysis using 66287-1-Ig (same clone as 66287-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66287-1-Ig (LDHA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66287-1-PBS in a different storage buffer formulation.

  • WB analysis of pig liver using 66287-1-Ig (same clone as 66287-1-PBS)

    WB analysis of pig liver using 66287-1-Ig (same clone as 66287-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66287-1-Ig (LDHA Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66287-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66287-1-Ig (same clone as 66287-1-PBS)

    IHC staining of human liver cancer using 66287-1-Ig (same clone as 66287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66287-1-Ig (LDHA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66287-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66287-1-Ig (same clone as 66287-1-PBS)

    IHC staining of human liver cancer using 66287-1-Ig (same clone as 66287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66287-1-Ig (LDHA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66287-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66287-1-Ig (same clone as 66287-1-PBS)

    IF Staining of HepG2 using 66287-1-Ig (same clone as 66287-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66287-1-Ig(LDHA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66287-1-PBS in a different storage buffer formulation.

产品信息

66287-1-PBS targets LDHA in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, pig, rat
Immunogen LDHA fusion protein Ag21417 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name lactate dehydrogenase A
Synonyms lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, PIG19
Calculated Molecular Weight 332 aa, 37 kDa
Observed Molecular Weight 32-37 kDa
GenBank Accession NumberBC067223
Gene Symbol LDHA
Gene ID (NCBI) 3939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00338
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies. Mutations in LDHA have been linked to exertional myoglobinuria. LDHA has some isoforms with MW 26-40 kDa.