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LDHA-Specific Recombinant antibody, PBS Only

LDHA-Specific Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240885A9

Cat no : 84198-2-PBS

Print datasheet

Synonyms

LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein

验证数据展示

  • WB analysis using 84198-2-RR (same clone as 84198-2-PBS)

    WB analysis using 84198-2-RR (same clone as 84198-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84198-2-RR (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84198-2-RR (same clone as 84198-2-PBS)

    FC experiment of HepG2 using 84198-2-RR (same clone as 84198-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LDHA-Specific Recombinant antibody (84198-2-RR, Clone:240885A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

产品信息

84198-2-PBS targets LDHA-Specific in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lactate dehydrogenase A
Synonyms LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein
Calculated Molecular Weight 37 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberNM_005566
Gene Symbol LDHA
Gene ID (NCBI) 3939
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP00338
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.