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验证数据展示

  • WB analysis using 84198-3-RR

    WB analysis using 84198-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84198-3-RR (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of MDA-MB-231 using 84198-3-RR

    WB analysis of MDA-MB-231 using 84198-3-RR

    WB result of LDHA-Specific antibody (84198-3-RR; 1:1000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells.

  • FC experiment of HepG2 using 84198-3-RR

    FC experiment of HepG2 using 84198-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LDHA-Specific Recombinant antibody (84198-3-RR, Clone:240885C7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证
View 3D epitope

LDHA-Specific Recombinant antibody

LDHA-Specific Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84198-3-RR

产品说明书

CloneNo. 240885C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated

规格: 
50 μL
100 μL
150 μL

¥2480/ 100 μL

浓度: 1000 μg/ml

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经过测试的应用

Positive WB detected inHeLa cells, MDA-MB-231 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84198-3-RR targets LDHA-Specific in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lactate dehydrogenase A
别名 LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein
计算分子量 37 kDa
观测分子量35 kDa
GenBank蛋白编号NM_005566
基因名称 LDHA
Gene ID (NCBI) 3939
RRIDAB_3671753
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP00338
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.

实验方案

Product Specific Protocols
WB protocol for LDHA-Specific antibody 84198-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols