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验证数据展示

  • WB analysis using 14546-1-AP

    WB analysis using 14546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14546-1-AP (LDHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of MDA-MB-231 using 14546-1-AP

    WB analysis of MDA-MB-231 using 14546-1-AP

    WB result of LDHC antibody (14546-1-AP; 1:800; room temperature for 1.5 hours) with wild-type and LDHC knockout MDA-MB-231 cells.

  • IHC staining of mouse testis using 14546-1-AP

    IHC staining of mouse testis using 14546-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14546-1-AP (LDHC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 14546-1-AP

    IHC staining of mouse testis using 14546-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14546-1-AP (LDHC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 14546-1-AP

    IF Staining of HeLa using 14546-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDHC antibody (14546-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

LDHC Polyclonal antibody

LDHC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14546-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

LDH-C, LDH3, LDH X, LDH C, LDH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MDA-MB-231 cells, PC-3 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14546-1-AP targets LDHC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LDHC fusion protein Ag6050 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lactate dehydrogenase C
别名 LDH-C, LDH3, LDH X, LDH C, LDH
计算分子量 36 kDa
观测分子量31-35 kDa
GenBank蛋白编号BC064388
基因名称 LDHC
Gene ID (NCBI) 3948
RRIDAB_3085437
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07864
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.

实验方案

Product Specific Protocols
WB protocol for LDHC antibody 14546-1-APDownload protocol
IHC protocol for LDHC antibody 14546-1-APDownload protocol
IF protocol for LDHC antibody 14546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols