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LDLR Recombinant antibody, PBS Only

LDLR Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1O11

Cat no : 82724-1-PBS

Print datasheet

Synonyms

LDL receptor, FHC, FH, 1O11

验证数据展示

  • WB analysis using 82724-1-RR (same clone as 82724-1-PBS)

    WB analysis using 82724-1-RR (same clone as 82724-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82724-1-RR (LDLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82724-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82724-1-RR (same clone as 82724-1-PBS)

    IHC staining of mouse brain using 82724-1-RR (same clone as 82724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82724-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82724-1-RR (same clone as 82724-1-PBS)

    IHC staining of mouse brain using 82724-1-RR (same clone as 82724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82724-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82724-1-RR (same clone as 82724-1-PBS)

    IF Staining of HeLa using 82724-1-RR (same clone as 82724-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDLR antibody (82724-1-RR, Clone: 1O11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82724-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82724-1-RR

    Affinity and Kinetic Characterization of 82724-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82724-1-RR against Human LDLR were performed. The affinity constant is below 1 pM.

产品信息

82724-1-PBS targets LDLR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LDLR fusion protein Ag1236 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name low density lipoprotein receptor
Synonyms LDL receptor, FHC, FH, 1O11
Calculated Molecular Weight 95 kDa
Observed Molecular Weight140-160 kDa
GenBank Accession NumberBC014514
Gene Symbol LDLR
Gene ID (NCBI) 3949
ENSEMBL Gene IDENSG00000130164
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01130
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.