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ARH Polyclonal antibody

ARH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13213-1-AP

Print datasheet

Synonyms

LDLRAP1, FHCB2, FHCB1, ARH2, ARH1

验证数据展示

  • WB analysis using 13213-1-AP

    WB analysis using 13213-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13213-1-AP (ARH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 13213-1-AP

    WB analysis of HeLa using 13213-1-AP

    WB result of ARH antibody (13213-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARH transfected HeLa cells.

  • IHC staining of human liver cancer using 13213-1-AP

    IHC staining of human liver cancer using 13213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13213-1-AP (ARH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 13213-1-AP

    IHC staining of human liver cancer using 13213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13213-1-AP (ARH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13213-1-AP targets ARH in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen ARH fusion protein Ag4006 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name low density lipoprotein receptor adaptor protein 1
Synonyms LDLRAP1, FHCB2, FHCB1, ARH2, ARH1
Calculated Molecular Weight 308 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC029770
Gene Symbol ARH
Gene ID (NCBI) 26119
RRIDAB_2135283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SW96
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARH antibody 13213-1-APDownload protocol
IHC protocol for ARH antibody 13213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep

A multienzyme S-nitrosylation cascade regulates cholesterol homeostasis

Authors - Colin T Stomberski
View Article
mouseWB

Cell Biol Toxicol

Activation of lipophagy ameliorates cadmium-induced neural tube defects via reducing low density lipoprotein cholesterol levels in mouse placentas

Authors - Yu-Feng Zhang
View Article