验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse uterus tissue |
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13991-1-AP targets LEFTY2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | LEFTY2 fusion protein Ag5073 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | left-right determination factor 2 |
| 别名 | Protein lefty-2, LEFTY 2, Left-right determination factor A, Left-right determination factor 2, left right determination factor 2 |
| 计算分子量 | 366 aa, 41 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC035718 |
| 基因名称 | LEFTY2 |
| Gene ID (NCBI) | 7044 |
| RRID | AB_2135782 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00292 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LEFTY2 antibody 13991-1-AP | Download protocol |
| IHC protocol for LEFTY2 antibody 13991-1-AP | Download protocol |
| IF protocol for LEFTY2 antibody 13991-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stroke Mesenchymal Behavior of the Endothelium Promoted by SMAD6 Downregulation Is Associated With Brain Arteriovenous Malformation Microhemorrhage. | ||
J Biol Chem MicroRNA-127 Promotes Mesendoderm Differentiation of Mouse Embryonic Stem Cells by Targeting Left-Right Determination Factor 2. | ||
Dev Biol Role of EBAF/Nodal/p27 signaling pathway in development of placenta in normal and diabetic rats. |
