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LEFTY2 Polyclonal antibody

LEFTY2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13991-1-AP

Print datasheet

Synonyms

Protein lefty-2, LEFTY 2, Left-right determination factor A, Left-right determination factor 2, left right determination factor 2

验证数据展示

  • WB analysis of HeLa using 13991-1-AP

    WB analysis of HeLa using 13991-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13991-1-AP (LEFTY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 13991-1-AP

    IHC staining of human endometrial cancer using 13991-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13991-1-AP (LEFTY2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human endometrial cancer using 13991-1-AP

    IHC staining of human endometrial cancer using 13991-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13991-1-AP (LEFTY2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 13991-1-AP

    IF Staining of HeLa using 13991-1-AP

    Immunofluorescent analysis of HeLa cells using 13991-1-AP (LEFTY2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse uterus tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13991-1-AP targets LEFTY2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen LEFTY2 fusion protein Ag5073 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name left-right determination factor 2
Synonyms Protein lefty-2, LEFTY 2, Left-right determination factor A, Left-right determination factor 2, left right determination factor 2
Calculated Molecular Weight 366 aa, 41 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC035718
Gene Symbol LEFTY2
Gene ID (NCBI) 7044
RRIDAB_2135782
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00292
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LEFTY2 antibody 13991-1-APDownload protocol
IHC protocol for LEFTY2 antibody 13991-1-APDownload protocol
IF protocol for LEFTY2 antibody 13991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Stroke

Mesenchymal Behavior of the Endothelium Promoted by SMAD6 Downregulation Is Associated With Brain Arteriovenous Malformation Microhemorrhage.

Authors - Weilun Fu
View Article
mouseWB

J Biol Chem

MicroRNA-127 Promotes Mesendoderm Differentiation of Mouse Embryonic Stem Cells by Targeting Left-Right Determination Factor 2.

Authors - Haixia Ma
View Article
ratIHC

Dev Biol

Role of EBAF/Nodal/p27 signaling pathway in development of placenta in normal and diabetic rats.

Authors - Qianqian Gao
View Article