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LEMD2 Polyclonal antibody

LEMD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29406-1-AP

Print datasheet

Synonyms

dJ482C21.1, hLEM2, LEM domain containing 2, LEMD2

验证数据展示

  • WB analysis using 29406-1-AP

    WB analysis using 29406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29406-1-AP (LEMD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 29406-1-AP

    IHC staining of human colon using 29406-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 29406-1-AP

    IHC staining of human colon using 29406-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, MCF-7 cells, U2OS cells, human testis tissue
Positive IHC detected inhuman colon tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29406-1-AP targets LEMD2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen LEMD2 fusion protein Ag28078 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LEM domain containing 2
Synonyms dJ482C21.1, hLEM2, LEM domain containing 2, LEMD2
Calculated Molecular Weight 503 aa, 57 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC051803
Gene Symbol LEMD2
Gene ID (NCBI) 221496
RRIDAB_2918298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NC56
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LEMD2 antibody 29406-1-APDownload protocol
IHC protocol for LEMD2 antibody 29406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols