• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

LENG9 Polyclonal antibody

LENG9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16295-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HeLa using 16295-1-AP

    WB analysis of HeLa using 16295-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16295-1-AP (LENG9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16295-1-AP

    IHC staining of human kidney using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 16295-1-AP

    IHC staining of human kidney using 16295-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16295-1-AP (LENG9 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 16295-1-AP

    IF Staining of HeLa using 16295-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LENG9 antibody (16295-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, Y79 cells
Positive IHC detected inhuman kidney tissue, human brain tissue, human heart tissue, human liver tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16295-1-AP targets LENG9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen LENG9 fusion protein Ag9481 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leukocyte receptor cluster (LRC) member 9
Synonyms
Calculated Molecular Weight 501 aa, 53 kDa
Observed Molecular Weight 54-60 kDa
GenBank Accession NumberBC015921
Gene Symbol LENG9
Gene ID (NCBI) 94059
RRIDAB_2296934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96B70
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LENG9 is rarely reported in the literature. GO function analysis revealed that the molecular function of LENG9 is metal ion binding (GO:0046872), and the BioGRID database showed that LENG9 has 6 interactors, including C9ORF41, B4GALT2, CDC5L, D2HGDH, FOXS1 and OGT. Studies have shown that LENG9 may play an oncogenic role in tumor pathogenesis by promoting tumor cell proliferation, migration, and invasion. (PMID: 35538075)

实验方案

Product Specific Protocols
WB protocol for LENG9 antibody 16295-1-APDownload protocol
IHC protocol for LENG9 antibody 16295-1-APDownload protocol
IF protocol for LENG9 antibody 16295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Long-read sequencing unveils high-resolution HPV integration and its oncogenic progression in cervical cancer.

Authors - Liyuan Zhou
View Article