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LEO1 Monoclonal antibody, PBS Only

LEO1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D7A2

Cat no : 68568-1-PBS

Print datasheet

Synonyms

LEO1, RDL

验证数据展示

  • WB analysis using 68568-1-Ig (same clone as 68568-1-PBS)

    WB analysis using 68568-1-Ig (same clone as 68568-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68568-1-Ig (LEO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68568-1-PBS in a different storage buffer formulation.

产品信息

68568-1-PBS targets LEO1 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen LEO1 fusion protein Ag33577 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Synonyms LEO1, RDL
Calculated Molecular Weight 666 aa, 75 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC018147
Gene Symbol LEO1
Gene ID (NCBI) 123169
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WVC0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LEO1, also named as RNA polymerase-associated protein LEO1, is a 666 amino acid protein, which belongs to the LEO1 family. LEO1 is a component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. The calculated molecular weight of LEO1 is 75 kDa, but the modified LEO1 protein is about 105 kDa (PMID: 15632063).