验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells, HEK-293 cells, mouse placenta tissue, mouse kidney tissue, human placenta tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human liver tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
15723-1-AP targets P3H2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | P3H2 fusion protein Ag8444 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leprecan-like 1 |
| 别名 | leprecan like 1, Leprecan like protein 1, LEPREL1, MLAT4, P3H2, Prolyl 3 hydroxylase 2 |
| 计算分子量 | 708aa,81 kDa; 527aa,60 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC005029 |
| 基因名称 | LEPREL1 |
| Gene ID (NCBI) | 55214 |
| RRID | AB_2136486 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IVL5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
P3H2 (prolyl 3-hydroxylase 2, also known as LEPREL1) is a member of the leprecan family of proteins, which also include P3H1, P3H3, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H2 shows prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types II, IV and V. Mutations in this gene are associated with nonsyndromic severe myopia with cataract and vitreoretinal degeneration, and downregulation of this gene may play a role in breast cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P3H2 antibody 15723-1-AP | Download protocol |
| IHC protocol for P3H2 antibody 15723-1-AP | Download protocol |
| IP protocol for P3H2 antibody 15723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Post-translationally abnormal collagens of prolyl 3-hydroxylase-2 null mice offer a pathobiological mechanism for the high myopia linked to human LEPREL1 mutations* | ||
Sci Rep Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure | ||
J Biol Chem Post-translational modification of type IV collagen with 3-hydroxyproline affects its interactions with glycoprotein VI and nidogens 1 and 2.
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