验证数据展示
经过测试的应用
| Positive WB detected in | mouse ovary tissue, MDA-MB-453s cells, rat ovary tissue |
| Positive IP detected in | MDA-MB-453 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16023-1-AP targets P3H3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | P3H3 fusion protein Ag8850 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leprecan-like 2 |
| 别名 | LEPREL2, Leprecan-like protein 2, Leprecan like protein 2, leprecan like 2, HSU47926 |
| 计算分子量 | 736 aa, 82 kDa |
| 观测分子量 | 82-90 kDa |
| GenBank蛋白编号 | BC017217 |
| 基因名称 | LEPREL2 |
| Gene ID (NCBI) | 10536 |
| RRID | AB_2281291 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IVL6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
P3H3 (prolyl 3-hydroxylase 3, also known as LEPREL2) is a member of the leprecan family of proteins, which also include P3H1, P3H2, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H3 is thought to reside in the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P3H3 antibody 16023-1-AP | Download protocol |
| IP protocol for P3H3 antibody 16023-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Genet Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation. | ||
PLoS Genet Cyclophilin B control of lysine post-translational modifications of skin type I collagen. | ||
Sci Rep Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome and endothelial function estimates. | ||
J Biol Chem P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.
| ||
J Biol Chem Type I and type V procollagen triple helix use different subsets of the molecular ensemble for lysine post-translational modifications in the rER. | ||
AIMS Genet Expression characterization and functional implication of the collagen-modifying Leprecan proteins in mouse gonadal tissue and mature sperm. |
