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- KD/KO Validated
LETM1 Polyclonal antibody
LETM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, Ramos cells |
Positive IHC detected in | human breast cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16024-1-AP targets LETM1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IF, CoIP |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Immunogen | LETM1 fusion protein Ag8861 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | leucine zipper-EF-hand containing transmembrane protein 1 |
Synonyms | LETM1 |
Calculated Molecular Weight | 739 aa, 83 kDa |
Observed Molecular Weight | 83 kDa |
GenBank Accession Number | BC021208 |
Gene Symbol | LETM1 |
Gene ID (NCBI) | 3954 |
RRID | AB_2136505 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O95202 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for LETM1 antibody 16024-1-AP | Download protocol |
IHC protocol for LETM1 antibody 16024-1-AP | Download protocol |
IF protocol for LETM1 antibody 16024-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature The mitochondrial Na(+)/Ca(2+) exchanger is essential for Ca(2+) homeostasis and viability. | ||
Sci Adv A common genetic variant of a mitochondrial RNA processing enzyme predisposes to insulin resistance. | ||
Nat Commun Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy. | ||
Proc Natl Acad Sci U S A Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome.
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Cell Rep The Mitochondrial Calcium Uniporter Matches Energetic Supply with Cardiac Workload during Stress and Modulates Permeability Transition. | ||
Genes Dis Aberrant LETM1 elevation dysregulates mitochondrial functions and energy metabolism and promotes lung metastasis in osteosarcoma
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