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  • Featured Product
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LETM1 Monoclonal antibody

LETM1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B9B9

Cat no : 68208-1-Ig

Print datasheet

Synonyms

LETM1

验证数据展示

  • WB analysis using 68208-1-Ig

    WB analysis using 68208-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68208-1-Ig (LETM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of rat heart using 68208-1-Ig

    WB analysis of rat heart using 68208-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 68208-1-Ig (LETM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68208-1-Ig

    IF Staining of HeLa using 68208-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of U-251 using 68208-1-Ig

    IF Staining of U-251 using 68208-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 68208-1-Ig

    IF Staining of HeLa using 68208-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LETM1 antibody (68208-1-Ig, Clone: 2B9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, rat heart tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells, U-251 cells
Planning an IHC experiment? We recommend our IHCeasy LETM1 Ready-To-Use IHC Kit. LETM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68208-1-Ig targets LETM1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, rat
Immunogen LETM1 fusion protein Ag8851 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name leucine zipper-EF-hand containing transmembrane protein 1
Synonyms LETM1
Calculated Molecular Weight 739 aa, 83 kDa
Observed Molecular Weight83 kDa
GenBank Accession NumberBC021208
Gene Symbol LETM1
Gene ID (NCBI) 3954
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO95202
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment.

实验方案

Product Specific Protocols
WB protocol for LETM1 antibody 68208-1-IgDownload protocol
IF protocol for LETM1 antibody 68208-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols