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验证数据展示

  • WB analysis of HepG2 using 23307-1-AP

    WB analysis of HepG2 using 23307-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23307-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 23307-1-AP

    IHC staining of human breast cancer using 23307-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 23307-1-AP

    IHC staining of human breast cancer using 23307-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 23307-1-AP

    IF Staining of HepG2 using 23307-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LGALS3BP antibody (23307-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

LGALS3BP Polyclonal antibody

LGALS3BP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23307-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

LG3BP, Lectin galactoside-binding soluble 3-binding protein, Galectin-3-binding protein, Galectin 3 binding protein, BTBD17B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23307-1-AP targets LGALS3BP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 LGALS3BP fusion protein Ag19700 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lectin, galactoside-binding, soluble, 3 binding protein
别名 LG3BP, Lectin galactoside-binding soluble 3-binding protein, Galectin-3-binding protein, Galectin 3 binding protein, BTBD17B
计算分子量 585 aa, 65 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC002403
基因名称 LGALS3BP
Gene ID (NCBI) 3959
RRIDAB_2879251
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08380
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).

实验方案

Product Specific Protocols
WB protocol for LGALS3BP antibody 23307-1-APDownload protocol
IHC protocol for LGALS3BP antibody 23307-1-APDownload protocol
IF protocol for LGALS3BP antibody 23307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols