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Galectin-4 Polyclonal antibody

Galectin-4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27552-1-AP

Print datasheet

Synonyms

Galectin 4, Gal-4, GAL4, Gal 4, Antigen NY-CO-27

验证数据展示

  • WB analysis using 27552-1-AP

    WB analysis using 27552-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27552-1-AP (Galectin-4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 27552-1-AP

    IHC staining of human stomach cancer using 27552-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27552-1-AP (Galectin-4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IHC staining of human colon cancer using 27552-1-AP

    IHC staining of human colon cancer using 27552-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IHC staining of human colon cancer using 27552-1-AP

    IHC staining of human colon cancer using 27552-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IF Staining of HT-29 using 27552-1-AP

    IF Staining of HT-29 using 27552-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Galectin-4 antibody (27552-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inrat colon tissue, mouse small intestine tissue, mouse colon tissue
Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy LGALS4 Ready-To-Use IHC Kit. LGALS4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

27552-1-AP targets Galectin-4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Galectin-4 fusion protein Ag26130 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lectin, galactoside-binding, soluble, 4
Synonyms Galectin 4, Gal-4, GAL4, Gal 4, Antigen NY-CO-27
Calculated Molecular Weight 323 aa, 36 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberBC034750
Gene Symbol Galectin-4
Gene ID (NCBI) 3960
RRIDAB_2880903
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56470
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.

实验方案

Product Specific Protocols
WB protocol for Galectin-4 antibody 27552-1-APDownload protocol
IHC protocol for Galectin-4 antibody 27552-1-APDownload protocol
IF protocol for Galectin-4 antibody 27552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

Single-Cell Transcriptomics Reveals Splicing Features of Adult Neural Stem Cells in the Subventricular Zone.

Authors - Yanlu Wang
View Article
humanWB

Cell Adh Migr

SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.

Authors - Yusuke Mizutani
View Article