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  • Featured Product
  • KD/KO Validated

LGMN Monoclonal antibody, PBS Only

LGMN Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B12

Cat no : 67017-1-PBS

Print datasheet

Synonyms

PRSC1, legumain, EC:3.4.22.34, 1F2B12

验证数据展示

  • WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LGMN antibody included
  • IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LGMN antibody included
  • WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 67017-1-Ig (same clone as 67017-1-PBS)

    WB analysis of MCF-7 using 67017-1-Ig (same clone as 67017-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LGMN antibody (67017-1-Ig, Clone: 1F2B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LGMN antibody (67017-1-Ig, Clone: 1F2B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy LGMN Ready-To-Use IHC Kit. LGMN primary antibody included.

产品信息

67017-1-PBS targets LGMN in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, pig
Immunogen LGMN fusion protein Ag7218 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name legumain
Synonyms PRSC1, legumain, EC:3.4.22.34, 1F2B12
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC003061
Gene Symbol LGMN
Gene ID (NCBI) 5641
RRIDAB_2882333
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99538
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).