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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LGMN antibody included
  • IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LGMN antibody included
  • WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 67017-1-Ig (same clone as 67017-1-PBS)

    WB analysis of MCF-7 using 67017-1-Ig (same clone as 67017-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LGMN antibody (67017-1-Ig, Clone: 1F2B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LGMN antibody (67017-1-Ig, Clone: 1F2B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

LGMN Monoclonal antibody, PBS Only

LGMN Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67017-1-PBS

产品说明书

CloneNo. 1F2B12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, pig

应用

WB, IHC, IF-P, Indirect ELISA

别名

PRSC1, legumain, EC:3.4.22.34, 1F2B12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy LGMN Ready-To-Use IHC Kit. LGMN primary antibody included.

产品信息

67017-1-PBS targets LGMN in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, pig
免疫原 LGMN fusion protein Ag7218 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 legumain
别名 PRSC1, legumain, EC:3.4.22.34, 1F2B12
计算分子量 49 kDa
观测分子量 36 kDa
GenBank蛋白编号BC003061
基因名称 LGMN
Gene ID (NCBI) 5641
RRIDAB_2882333
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99538
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).