验证数据展示
经过测试的应用
| Positive IHC detected in | human small intestine tissue, human colon tissue, human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21833-1-AP targets LGR5 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | LGR5 fusion protein Ag16389 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leucine-rich repeat-containing G protein-coupled receptor 5 |
| 别名 | FEX, G protein coupled receptor 49, G protein coupled receptor 67, GPR49, GPR67, GRP49, HG38, LGR5 |
| 计算分子量 | 907 aa, 100 kDa |
| GenBank蛋白编号 | BC096324 |
| 基因名称 | LGR5 |
| Gene ID (NCBI) | 8549 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75473 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LGR5, also named as GPR49, GPR67 and HG38, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. LGR5 is a stem cell marker of the intestinal epithelium and the hair follicule. It is a target gene of Wnt signaling.
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget LGR5 regulates pro-survival MEK/ERK and proliferative Wnt/β-catenin signalling in neuroblastoma. | ||
Inflamm Bowel Dis Overexpression of Cancer-Associated Stem Cell Gene OLFM4 in the Colonic Epithelium of Patients With Primary Sclerosing Cholangitis. | ||
J Dermatol Sci REGγ regulates hair cycle by activating Lgr5 positive hair follicle stem cells. | ||
Tumour Biol Correlation of Musashi-1, Lgr5, and pEGFR expressions in human small intestinal adenocarcinomas. | ||
Oncol Lett Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement. | ||
Oncol Lett Expression and clinical significance of SALL4 and LGR5 in patients with lung cancer. |
