验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, PC-3 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12840-1-AP targets LGTN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, hamster |
| 免疫原 | LGTN fusion protein Ag3579 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ligatin |
| 别名 | ligatin, Hepatocellular carcinoma-associated antigen 56, HCA56, EIF2D |
| 计算分子量 | 584 aa, 65 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC001585 |
| 基因名称 | LGTN |
| Gene ID (NCBI) | 1939 |
| RRID | AB_2135302 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P41214 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LGTN antibody 12840-1-AP | Download protocol |
| IHC protocol for LGTN antibody 12840-1-AP | Download protocol |
| IF protocol for LGTN antibody 12840-1-AP | Download protocol |
| IP protocol for LGTN antibody 12840-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.
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Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. | ||
Genes Dev Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling. | ||
Nat Commun A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation.
| ||
Nucleic Acids Res Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codons.
| ||
