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验证数据展示

  • WB analysis of Jurkat using 18133-1-AP

    WB analysis of Jurkat using 18133-1-AP

    WB result of LHX2 antibody (18133-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LHX2 transfected Jurkat cells.

  • WB analysis using 18133-1-AP

    WB analysis using 18133-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18133-1-AP (LHX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

LHX2 Polyclonal antibody

LHX2 Polyclonal Antibody for WB, ELISA
Cat No. 18133-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

hLhx2, Homeobox protein LH 2, LH2, LHX2, LIM homeobox 2, LIM homeobox protein 2, LIM/homeobox protein Lhx2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, Daudi cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18133-1-AP targets LHX2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LHX2 fusion protein Ag12717 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM homeobox 2
别名 hLhx2, Homeobox protein LH 2, LH2, LHX2, LIM homeobox 2, LIM homeobox protein 2, LIM/homeobox protein Lhx2
计算分子量 406 aa, 44 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC093662
基因名称 LHX2
Gene ID (NCBI) 9355
RRIDAB_3085555
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50458
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LHX proteins can activate or repress gene transcription by direct DNA binding and association with co-activators or co-repressors through their LIM domain. The LIM homeobox 2 (LHX2), also known as LH2, is a member of the LIM homeodomain family of proteins. LHX2 functions as a nuclear transcription co-factor and is reported to be involved in many important physiological processes, such as cell division, cell proliferation, specific cell-type differentiation, and cerebral cortex development. LHX2 promotes the growth and metastasis of nasopharyngeal carcinoma by regulating Wnt signaling and plays a vital role in breast cancer's progression and prognosis(PMID: 37345110).

实验方案

Product Specific Protocols
WB protocol for LHX2 antibody 18133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols