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LHX2 Polyclonal antibody

LHX2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18133-1-AP

Print datasheet

Synonyms

hLhx2, Homeobox protein LH 2, LH2, LHX2, LIM homeobox 2, LIM homeobox protein 2, LIM/homeobox protein Lhx2

验证数据展示

  • WB analysis of Jurkat using 18133-1-AP

    WB analysis of Jurkat using 18133-1-AP

    WB result of LHX2 antibody (18133-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LHX2 transfected Jurkat cells.

  • WB analysis using 18133-1-AP

    WB analysis using 18133-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18133-1-AP (LHX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, Daudi cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18133-1-AP targets LHX2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LHX2 fusion protein Ag12717 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LIM homeobox 2
Synonyms hLhx2, Homeobox protein LH 2, LH2, LHX2, LIM homeobox 2, LIM homeobox protein 2, LIM/homeobox protein Lhx2
Calculated Molecular Weight 406 aa, 44 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC093662
Gene Symbol LHX2
Gene ID (NCBI) 9355
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50458
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LHX proteins can activate or repress gene transcription by direct DNA binding and association with co-activators or co-repressors through their LIM domain. The LIM homeobox 2 (LHX2), also known as LH2, is a member of the LIM homeodomain family of proteins. LHX2 functions as a nuclear transcription co-factor and is reported to be involved in many important physiological processes, such as cell division, cell proliferation, specific cell-type differentiation, and cerebral cortex development. LHX2 promotes the growth and metastasis of nasopharyngeal carcinoma by regulating Wnt signaling and plays a vital role in breast cancer's progression and prognosis(PMID: 37345110).

实验方案

Product Specific Protocols
WB protocol for LHX2 antibody 18133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols