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验证数据展示

  • WB analysis using 31796-1-AP (same clone as 31796-1-PBS)

    WB analysis using 31796-1-AP (same clone as 31796-1-PBS)

    HEK-293T cells, HeLa cells, mouse kidney tissue, mouse liver tissue, mouse lung tissue, mouse spleen tissue lysates were subjected to SDS PAGE followed by western blot with 31796-1-AP (LIAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31796-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 31796-1-AP (same clone as 31796-1-PBS)

    IF Staining of HEK-293 using 31796-1-AP (same clone as 31796-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using LIAT1 antibody (31796-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31796-1-PBS in a different storage buffer formulation.

LIAT1 Polyclonal antibody, PBS Only

LIAT1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31796-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

Protein LIAT1, Ligand of ATE1 protein, C17orf97

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

31796-1-PBS targets LIAT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 LIAT1 fusion protein Ag37082 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 17 open reading frame 97
别名 Protein LIAT1, Ligand of ATE1 protein, C17orf97
观测分子量20-70 kDa
GenBank蛋白编号BC141806
基因名称 C17orf97
Gene ID (NCBI) 400566
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6ZQX7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Liat1 (Ligand of Ate1) is a protein of unknown function that was originally discovered through its interaction with arginyl tRNA protein transferase 1 (Ate1), a component of the Arg/N-degron pathway of protein degradation.Liat1 participates in Liquid-liquid phase separation (LLPS), a process which enables the temporal and spatial regulation of important cellular processes such as RNA metbolism, genome organization, DNA repair, and transcription regulation. Liat1 interacts with itself, which means Liat1 can assemble into different species in nucleus or cytosol. Our WB is consistent with Arva A et al (PMID: 33443146).