验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue, mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse embryo tissue |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26757-1-AP targets LIF in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | LIF fusion protein Ag25076 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leukemia inhibitory factor (cholinergic differentiation factor) |
| 别名 | Leukemia inhibitory factor, Melanoma-derived LPL inhibitor, Differentiation-stimulating factor |
| 计算分子量 | 202 aa, 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC093733 |
| 基因名称 | LIF |
| Gene ID (NCBI) | 3976 |
| RRID | AB_2880624 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P15018 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LIF antibody 26757-1-AP | Download protocol |
| IHC protocol for LIF antibody 26757-1-AP | Download protocol |
| IF protocol for LIF antibody 26757-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell LIF signaling regulates outer radial glial to interneuron fate during human cortical development | ||
Toxicology Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice | ||
Hum Reprod The depot GnRH agonist protocol improves the live birth rate per fresh embryo transfer cycle, but not the cumulative live birth rate in normal responders: a randomized controlled trial and molecular mechanism study. | ||
Int J Cancer Leukemia inhibitory factor is a novel biomarker to predict lymph node and distant metastasis in pancreatic cancer. | ||
Reprod Biol Endocrinol Expression and significance of miR-30d-5p and SOCS1 in patients with recurrent implantation failure during implantation window | ||
Reproduction Consistency and synchronization of AMPK-glycogen in endometrial epithelial cells are critical to the embryo implantation. |
