• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

LIFR Polyclonal antibody

LIFR Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 22779-1-AP

Print datasheet

Synonyms

STWS, SJS2, LIF-R, LIF receptor, LIF R

验证数据展示

  • WB analysis of human skeletal muscle using 22779-1-AP

    WB analysis of human skeletal muscle using 22779-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22779-1-AP (LIFR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 22779-1-AP

    IHC staining of mouse skeletal muscle using 22779-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22779-1-AP (LIFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 22779-1-AP

    IHC staining of mouse cerebellum using 22779-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22779-1-AP (LIFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skeletal muscle using 22779-1-AP

    IHC staining of human skeletal muscle using 22779-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22779-1-AP (LIFR Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skeletal muscle using 22779-1-AP

    IHC staining of human skeletal muscle using 22779-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22779-1-AP (LIFR Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 22779-1-AP

    IF Staining of HeLa using 22779-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LIFR antibody (22779-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IP experiment of mouse skeletal muscle using 22779-1-AP

    IP experiment of mouse skeletal muscle using 22779-1-AP

    IP result of anti-LIFR (IP:22779-1-AP, 4ug; Detection:22779-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

  • IF Staining of mouse cerebellum using 22779-1-AP

    IF Staining of mouse cerebellum using 22779-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using LIFR antibody (22779-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 22779-1-AP

    IF Staining of mouse cerebellum using 22779-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using LIFR antibody (22779-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue, human kidney tissue, human placenta tissue, mouse cerebellum tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22779-1-AP targets LIFR in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen LIFR fusion protein Ag18742 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leukemia inhibitory factor receptor alpha
Synonyms STWS, SJS2, LIF-R, LIF receptor, LIF R
Calculated Molecular Weight 1097 aa, 124 kDa
Observed Molecular Weight 190 kDa
GenBank Accession NumberBC153096
Gene Symbol LIFR
Gene ID (NCBI) 3977
RRIDAB_2879165
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP42702
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIFR, also known as CD118, is a subunit of a receptor for leukemia inhibitory factor (LIF). LIF is a pleiotropic cytokine of the interleukin-6 family which affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIFR is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of LIF. The high-affinity complex also binds a related cytokine, oncostatin M (PMID: 8999038). LIFR has also been identified as a breast cancer metastasis suppressor that functions through the HIPPO-YAP pathway (PMID: 23001183).

实验方案

Product Specific Protocols
WB protocol for LIFR antibody 22779-1-APDownload protocol
IHC protocol for LIFR antibody 22779-1-APDownload protocol
IF protocol for LIFR antibody 22779-1-APDownload protocol
IP protocol for LIFR antibody 22779-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Nature

Exercise plasma boosts memory and dampens brain inflammation via clusterin.

Authors - Zurine De Miguel
View Article
mouseWB,IF

Nat Metab

LIFR regulates cholesterol-driven bidirectional hepatocyte-neutrophil cross-talk to promote liver regeneration

Authors - Yalan Deng
View Article
mouseWB

Nat Commun

A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis.

Authors - Fan Yao
  • KO Validated
View Article
humanWB

Clin Transl Med

Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.

Authors - An-Dong Liu
View Article
humanWB,IHC,IF

Hum Mol Genet

Mutations in the leukemia inhibitory factor receptor (LIFR) gene and Lifr deficiency cause urinary tract malformations.

Authors - Anne Kosfeld
View Article
humanWB

J Exp Clin Cancer Res

miR-589 promotes gastric cancer aggressiveness by a LIFR-PI3K/AKT-c-Jun regulatory feedback loop.

Authors - Feifei Zhang
View Article