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LIG1 Monoclonal antibody

LIG1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3D9G8

Cat no : 67840-1-Ig

Print datasheet

Synonyms

DNA ligase 1, DNA ligase I, LIG1, ligase I, DNA, ATP dependent

验证数据展示

  • WB analysis using 67840-1-Ig

    WB analysis using 67840-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67840-1-Ig (LIG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, MOLT-4 cells, K-562 cells, HSC-T6 cells
Planning an IHC experiment? We recommend our IHCeasy LIG1 Ready-To-Use IHC Kit. LIG1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67840-1-Ig targets LIG1 in WB, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen LIG1 fusion protein Ag12489 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ligase I, DNA, ATP-dependent
Synonyms DNA ligase 1, DNA ligase I, LIG1, ligase I, DNA, ATP dependent
Calculated Molecular Weight 919 aa, 102 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC108318
Gene Symbol LIG1
Gene ID (NCBI) 3978
RRIDAB_2918602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18858
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 67840-1-Ig is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.

实验方案

Product Specific Protocols
WB protocol for LIG1 antibody 67840-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

bioRxiv

Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy

Authors - Zhihua Kang
View Article