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DNA ligase III/LIG3 Polyclonal antibody

DNA ligase III/LIG3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 26583-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

LIG3, ligase III, DNA, ATP dependent, LIG2, EC:6.5.1.1, DNA ligase III

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, PC-3 cells
Positive IP detected inHEK-293T cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LIG3 Ready-To-Use IHC Kit. LIG3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26583-1-AP targets DNA ligase III/LIG3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, pig
免疫原 DNA ligase III/LIG3 fusion protein Ag24998 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ligase III, DNA, ATP-dependent
别名 LIG3, ligase III, DNA, ATP dependent, LIG2, EC:6.5.1.1, DNA ligase III
计算分子量 100-110 kDa
观测分子量 100 kDa
GenBank蛋白编号BC068005
基因名称 LIG3
Gene ID (NCBI) 3980
RRIDAB_2880562
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49916
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA ligase III(DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.

实验方案

Product Specific Protocols
WB protocol for DNA ligase III/LIG3 antibody 26583-1-APDownload protocol
IHC protocol for DNA ligase III/LIG3 antibody 26583-1-APDownload protocol
IF protocol for DNA ligase III/LIG3 antibody 26583-1-APDownload protocol
IP protocol for DNA ligase III/LIG3 antibody 26583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Brain

Biallelic variants in LIG3 cause a novel mitochondrial neurogastrointestinal encephalomyopathy.

Authors - Elena Bonora
pigWB

Int J Biochem Cell Biol

Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.

Authors - Guoling Li
humanWB

bioRxiv

Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy

Authors - Zhihua Kang
WB

bioRxiv

D-type cyclins regulate DNA mismatch repair in the G1 and S phases of the cell cycle, maintaining genome stability

Authors - Gergely Rona
humanWB

Mol Cell

CDK-independent role of D-type cyclins in regulating DNA mismatch repair

Authors - Gergely Rona
humanWB

Nature

Retrotransposons hijack alt-EJ for DNA replication and eccDNA biogenesis

Authors - Fu Yang
  • KO Validated
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