验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, mouse liver tissue, HepG2 cells, HeLa cells, rat testis tissue |
| Positive IHC detected in | human prostate cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12695-1-AP targets LIG4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | LIG4 fusion protein Ag3385 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ligase IV, DNA, ATP-dependent |
| Synonyms | DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent |
| Calculated Molecular Weight | 911 aa, 104 kDa |
| Observed Molecular Weight | 100-104 kDa |
| GenBank Accession Number | BC037491 |
| Gene Symbol | LIG4 |
| Gene ID (NCBI) | 3981 |
| RRID | AB_2136253 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49917 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LIG4 antibody 12695-1-AP | Download protocol |
| IHC protocol for LIG4 antibody 12695-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Host Microbe Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication.
| ||
Nat Commun Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling. | ||
J Allergy Clin Immunol XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiency. | ||
Nat Commun Rap1 regulates hematopoietic stem cell survival and affects oncogenesis and response to chemotherapy. | ||
Nat Commun Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway. | ||
Cancer Res Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining. |
