验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, mouse liver tissue, HepG2 cells, HeLa cells, rat testis tissue |
| Positive IHC detected in | human prostate cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12695-1-AP targets LIG4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | LIG4 fusion protein Ag3385 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ligase IV, DNA, ATP-dependent |
| 别名 | DNA ligase 4, DNA ligase IV, LIG4, ligase IV, DNA, ATP dependent |
| 计算分子量 | 911 aa, 104 kDa |
| 观测分子量 | 100-104 kDa |
| GenBank蛋白编号 | BC037491 |
| 基因名称 | LIG4 |
| Gene ID (NCBI) | 3981 |
| RRID | AB_2136253 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49917 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LIG4 antibody 12695-1-AP | Download protocol |
| IHC protocol for LIG4 antibody 12695-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Host Microbe Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication.
| ||
Nat Commun Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling. | ||
J Allergy Clin Immunol XRCC4 deficiency in human subjects causes a marked neurological phenotype but no overt immunodeficiency. | ||
Nat Commun Rap1 regulates hematopoietic stem cell survival and affects oncogenesis and response to chemotherapy. | ||
Nat Commun Interactome analysis identifies a new paralogue of XRCC4 in non-homologous end joining DNA repair pathway. | ||
Cancer Res Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining. |
