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  • Featured Product
  • KD/KO Validated

LIG4 Polyclonal antibody, PBS Only

LIG4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12695-1-PBS

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Synonyms

验证数据展示

  • WB analysis of mouse testis using 12695-1-AP (same clone as 12695-1-PBS)

    WB analysis of mouse testis using 12695-1-AP (same clone as 12695-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 12695-1-AP (same clone as 12695-1-PBS)

    IHC staining of human prostate cancer using 12695-1-AP (same clone as 12695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12695-1-AP (LIG4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

产品信息

12695-1-PBS targets LIG4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LIG4 fusion protein Ag3385 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ligase IV, DNA, ATP-dependent
Synonyms
Calculated Molecular Weight 911 aa, 104 kDa
Observed Molecular Weight 100-104 kDa
GenBank Accession NumberBC037491
Gene Symbol LIG4
Gene ID (NCBI) 3981
RRIDAB_2136253
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49917
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.