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验证数据展示

  • WB analysis of MCF-7 using 67119-1-Ig

    WB analysis of MCF-7 using 67119-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67119-1-Ig (LILRB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 67119-1-Ig

    IF Staining of A549 using 67119-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using LILRB4 antibody (67119-1-Ig, Clone: 1C8E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

ILT-3/LILRB4 Monoclonal antibody

ILT-3/LILRB4 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67119-1-Ig

Print datasheet

CloneNo. 1C8E1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

LILRB4, Immunoglobulin-like transcript 3, ILT-3, ILT3, CD85k

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, A549 cells, HUVEC cells
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67119-1-Ig targets ILT-3/LILRB4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ILT-3/LILRB4 fusion protein Ag3219 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4
Synonyms LILRB4, Immunoglobulin-like transcript 3, ILT-3, ILT3, CD85k
Calculated Molecular Weight 448 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC026309
Gene Symbol LILRB4
Gene ID (NCBI) 11006
RRIDAB_2882422
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NHJ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ILT-3/LILRB4 antibody 67119-1-IgDownload protocol
IF protocol for ILT-3/LILRB4 antibody 67119-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols