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验证数据展示

  • WB analysis of MCF-7 using 67119-1-Ig

    WB analysis of MCF-7 using 67119-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67119-1-Ig (LILRB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 67119-1-Ig

    IF Staining of A549 using 67119-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using LILRB4 antibody (67119-1-Ig, Clone: 1C8E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

ILT-3/LILRB4 Monoclonal antibody

ILT-3/LILRB4 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67119-1-Ig

产品说明书

CloneNo. 1C8E1

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

LILRB4, Immunoglobulin-like transcript 3, ILT-3, ILT3, CD85k

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, A549 cells, HUVEC cells
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67119-1-Ig targets ILT-3/LILRB4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ILT-3/LILRB4 fusion protein Ag3219 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4
别名 LILRB4, Immunoglobulin-like transcript 3, ILT-3, ILT3, CD85k
计算分子量 448 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC026309
基因名称 LILRB4
Gene ID (NCBI) 11006
RRIDAB_2882422
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NHJ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ILT-3/LILRB4 antibody 67119-1-IgDownload protocol
IF protocol for ILT-3/LILRB4 antibody 67119-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols