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  • Featured Product
  • KD/KO Validated

LIMD2 Polyclonal antibody

LIMD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15471-1-AP

Print datasheet

Synonyms

LIM domain containing 2, LIMD2

验证数据展示

  • WB analysis of U-937 using 15471-1-AP

    WB analysis of U-937 using 15471-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 15471-1-AP (LIMD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 15471-1-AP

    IHC staining of human liver cancer using 15471-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15471-1-AP (LIMD2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 15471-1-AP

    IHC staining of human liver cancer using 15471-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15471-1-AP (LIMD2 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inU-937 cells, Raji cells
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15471-1-AP targets LIMD2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen LIMD2 fusion protein Ag7775 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LIM domain containing 2
Synonyms LIM domain containing 2, LIMD2
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC004400
Gene Symbol LIMD2
Gene ID (NCBI) 80774
RRIDAB_10859817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BT23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LIMD2 antibody 15471-1-APDownload protocol
IHC protocol for LIMD2 antibody 15471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Bioengineered

LIM domain-containing 2 (LIMD2) promotes the progress of ovarian cancer via the focal adhesion signaling pathway.

Authors - Lixin Chen
View Article
humanWB

Mol Biol Rep

Knockdown of LIMD2 inhibits the progression of ovarian carcinoma through ERK1/2 pathway

Authors - Haiyang Hu
  • KD Validated
View Article