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LIN41 Polyclonal antibody

LIN41 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55003-1-AP

Print datasheet

Synonyms

TRIM71, RING-type E3 ubiquitin transferase TRIM71, Protein lin-41 homolog, Protein lin 41 homolog, LIN 41

验证数据展示

  • WB analysis of mouse kidney using 55003-1-AP

    WB analysis of mouse kidney using 55003-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55003-1-AP (LIN41 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 55003-1-AP

    IHC staining of human testis using 55003-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 55003-1-AP (LIN41 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inmouse kidney tissue, DU 145 cells, NIH/3T3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55003-1-AP targets LIN41 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 71
Synonyms TRIM71, RING-type E3 ubiquitin transferase TRIM71, Protein lin-41 homolog, Protein lin 41 homolog, LIN 41
Calculated Molecular Weight 93 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberNM_001039111
Gene Symbol LIN41
Gene ID (NCBI) 131405
RRIDAB_2616313
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2Q1W2
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM71, also named as LIN41, belongs to the TRIM/RBCC family. It may be involved in controlling the timing of organ formation during development. The antibody is specific to TRIM71.

实验方案

Product Specific Protocols
WB protocol for LIN41 antibody 55003-1-APDownload protocol
IHC protocol for LIN41 antibody 55003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Cycle

TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs.

Authors - Daniel J Foster
View Article
humanWB

Cell Mol Gastroenterol Hepatol

HDLBP promotes hepatocellular carcinoma proliferation and sorafenib resistance by suppressing Trim71-dependent RAF1 degradation

Authors - Jingsheng Yuan
View Article
human,mouseIHC

Mol Carcinog

Circ_0007429/miR-637/TRIM71/Ago2 axis participates in the regulation of proliferation, migration, invasion, apoptosis, and aerobic glycolysis of HCC

Authors - Lilan Fan
View Article