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LIN7A Polyclonal antibody

LIN7A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25150-1-AP

Print datasheet

Synonyms

hLin 7, lin 7 homolog A (C. elegans), LIN 7A, LIN7, LIN7A, MALS 1, MALS1, Mammalian lin seven protein 1, Protein lin 7 homolog A, Tax interaction protein 33, TIP 33, Veli 1, VELI1, Vertebrate lin 7 homolog 1

验证数据展示

  • WB analysis of rat brain using 25150-1-AP

    WB analysis of rat brain using 25150-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25150-1-AP (LIN7A Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • IP experiment of mouse brain using 25150-1-AP

    IP experiment of mouse brain using 25150-1-AP

    IP result of anti-LIN7A (IP:25150-1-AP, 3ug; Detection:25150-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of mouse brain using 25150-1-AP

    IHC staining of mouse brain using 25150-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25150-1-AP (LIN7A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse brain using 25150-1-AP

    IHC staining of mouse brain using 25150-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25150-1-AP (LIN7A Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inrat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of 25150-1-AP is LIN7A Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen LIN7A fusion protein Ag18440 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lin-7 homolog A (C. elegans)
Synonyms hLin 7, lin 7 homolog A (C. elegans), LIN 7A, LIN7, LIN7A, MALS 1, MALS1, Mammalian lin seven protein 1, Protein lin 7 homolog A, Tax interaction protein 33, TIP 33, Veli 1, VELI1, Vertebrate lin 7 homolog 1
Calculated Molecular Weight 233 aa, 26 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC118609
Gene Symbol LIN7A
Gene ID (NCBI) 8825
RRIDAB_2879925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14910
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LIN7A antibody 25150-1-APDownload protocol
IHC protocol for LIN7A antibody 25150-1-APDownload protocol
IP protocol for LIN7A antibody 25150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

iScience

Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration

Authors - Haibin Tian
View Article