验证数据展示
经过测试的应用
| Positive WB detected in | mouse adipose tissue, pig adipose tissue |
| Positive IHC detected in | mouse brown adipose tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17333-1-AP targets HSL in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, pig |
| 文献引用反应性 | human, mouse, rat, pig, bovine |
| 免疫原 | HSL fusion protein Ag11360 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lipase, hormone-sensitive |
| 别名 | LIPE, REH, LHS, Hormone-sensitive lipase, EC:3.1.1.79 |
| 计算分子量 | 117 kDa |
| 观测分子量 | 84 kDa |
| GenBank蛋白编号 | BC070041 |
| 基因名称 | HSL |
| Gene ID (NCBI) | 3991 |
| RRID | AB_2878386 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q05469 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSL antibody 17333-1-AP | Download protocol |
| IHC protocol for HSL antibody 17333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf Paternal cadmium exposure affects testosterone synthesis by reducing the testicular cholesterol pool in offspring mice | ||
Int J Biol Sci LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis. | ||
Biochim Biophys Acta Mol Cell Biol Lipids DECR1 directly activates HSL to promote lipolysis in cervical cancer cells. | ||
Biochim Biophys Acta Mol Cell Biol Lipids PLK1 promotes cholesterol efflux and alleviates atherosclerosis by up-regulating ABCA1 and ABCG1 expression via the AMPK/PPARγ/LXRα pathway | ||
Epigenetics Differential regulatory roles of microRNAs during intramuscular adipogenesis in Chinese Guizhou Congjiang Xiang pigs. | ||
Food Funct Gentisic acid prevents diet-induced obesity in mice by accelerating the thermogenesis of brown adipose tissue. |
