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验证数据展示

  • WB analysis of MKN-45 using 25889-1-AP

    WB analysis of MKN-45 using 25889-1-AP

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 25889-1-AP

    IHC staining of human stomach using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human stomach using 25889-1-AP

    IHC staining of human stomach using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 25889-1-AP

    WB analysis using 25889-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

LIPF Polyclonal antibody

LIPF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25889-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

lipase, gastric, HLAL, HGL, GL, Gastric lipase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMKN-45 cells, mouse stomach tissue
Positive IHC detected inhuman stomach tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25889-1-AP targets LIPF in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 LIPF fusion protein Ag23149 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lipase, gastric
别名 lipase, gastric, HLAL, HGL, GL, Gastric lipase
观测分子量 49 kDa
GenBank蛋白编号BC112272
基因名称 LIPF
Gene ID (NCBI) 8513
RRIDAB_2880284
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07098
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

gastric lipase, is an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions.

实验方案

Product Specific Protocols
WB protocol for LIPF antibody 25889-1-APDownload protocol
IHC protocol for LIPF antibody 25889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols