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LIPF Polyclonal antibody

LIPF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25889-1-AP

Print datasheet

Synonyms

lipase, gastric, HLAL, HGL, GL, Gastric lipase

验证数据展示

  • WB analysis of MKN-45 using 25889-1-AP

    WB analysis of MKN-45 using 25889-1-AP

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 25889-1-AP

    IHC staining of human stomach using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human stomach using 25889-1-AP

    IHC staining of human stomach using 25889-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 25889-1-AP

    WB analysis using 25889-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMKN-45 cells, mouse stomach tissue
Positive IHC detected inhuman stomach tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25889-1-AP targets LIPF in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen LIPF fusion protein Ag23149 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lipase, gastric
Synonyms lipase, gastric, HLAL, HGL, GL, Gastric lipase
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC112272
Gene Symbol LIPF
Gene ID (NCBI) 8513
RRIDAB_2880284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07098
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

gastric lipase, is an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions.

实验方案

Product Specific Protocols
WB protocol for LIPF antibody 25889-1-APDownload protocol
IHC protocol for LIPF antibody 25889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols