验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, COLO 320 cells, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16602-1-AP targets LIPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | LIPH fusion protein Ag9775 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lipase, member H |
| 别名 | LPDLR, LPD lipase-related protein, lipase, member H, Lipase member H, EC:3.1.1.- |
| 计算分子量 | 51 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC064941 |
| 基因名称 | LIPH |
| Gene ID (NCBI) | 200879 |
| RRID | AB_2135352 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WWY8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LIPH(Lipase member H) is also named as LPDLR, MPAPLA1, PLA1B. It is a phosphatidic acid-selective phospholipase A1 (PLA1) that produces 2-acyl lysophosphatidic acid (LPA) and belongs to the AB hydrolase superfamily and Lipase family.LIPH has a 12-residue lid region, which likely covers a catalytic pocket, and 4 potential N-linked glycosylation sites. Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LIPH antibody 16602-1-AP | Download protocol |
| IHC protocol for LIPH antibody 16602-1-AP | Download protocol |
| IF protocol for LIPH antibody 16602-1-AP | Download protocol |
| IP protocol for LIPH antibody 16602-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genet Med Loss-of-function variants in C3ORF52 result in localized autosomal recessive hypotrichosis. | ||
Cell Oncol (Dordr) Genomic analysis and filtration of novel prognostic biomarkers based on metabolic and immune subtypes in pancreatic cancer | ||
J Cell Mol Med LIPH promotes metastasis by enriching stem-like cells in triple-negative breast cancer.
| ||
Tumour Biol Lipase member H is a novel secreted protein associated with a poor prognosis for breast cancer patients. | ||
Biochem Biophys Res Commun Lipase member H is a novel secreted protein selectively upregulated in human lung adenocarcinomas and bronchioloalveolar carcinomas. | ||
Tumour Biol Lipase member H frequently overexpressed in human esophageal adenocarcinomas. |
