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验证数据展示

  • WB analysis of SW 1990 using 66303-1-Ig

    WB analysis of SW 1990 using 66303-1-Ig

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 66303-1-Ig (LIPH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 66303-1-Ig

    IHC staining of human pancreas using 66303-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 10x lens).

  • IHC staining of human pancreas using 66303-1-Ig

    IHC staining of human pancreas using 66303-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66303-1-Ig (LIPH Antibody) at dilution of 1:500 (under 40x lens).

  • IF Staining of BxPC-3 using 66303-1-Ig

    IF Staining of BxPC-3 using 66303-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 66303-1-Ig(LIPH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

LIPH Monoclonal antibody

LIPH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66303-1-Ig

产品说明书

CloneNo. 1F5D10
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

AH, Lipase member H, lipase, member H, LIPH, LPD lipase related protein, LPDLR, mPA PLA1, mPA PLA1 alpha, MPAPLA1, Phospholipase A1 member B, PLA1B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSW 1990 cells, BxPC-3 cells, COLO 320 cells
Positive IHC detected inhuman pancreas tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

66303-1-Ig targets LIPH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, pig
文献引用反应性human
免疫原 LIPH fusion protein Ag24009 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 lipase, member H
别名 AH, Lipase member H, lipase, member H, LIPH, LPD lipase related protein, LPDLR, mPA PLA1, mPA PLA1 alpha, MPAPLA1, Phospholipase A1 member B, PLA1B
计算分子量 51 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC064941
基因名称 LIPH
Gene ID (NCBI) 200879
RRIDAB_2881686
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8WWY8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIPH(Lipase member H) is also named as LPDLR, MPAPLA1, PLA1B. It is a phosphatidic acid-selective phospholipase A1 (PLA1) that produces 2-acyl lysophosphatidic acid (LPA) and belongs to the AB hydrolase superfamily and Lipase family.LIPH has a 12-residue lid region, which likely covers a catalytic pocket, and 4 potential N-linked glycosylation sites. Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7).

实验方案

Product Specific Protocols
WB protocol for LIPH antibody 66303-1-IgDownload protocol
IHC protocol for LIPH antibody 66303-1-IgDownload protocol
IF protocol for LIPH antibody 66303-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Genet Med

Loss-of-function variants in C3ORF52 result in localized autosomal recessive hypotrichosis.

Authors - Liron Malki
View Article