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LIPI Polyclonal antibody

LIPI Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24300-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 17, CT17, Lipase member I, lipase, member I, LIPI, LPD lipase, LPDL, mPA PLA1 beta, PRED5

验证数据展示

  • WB analysis of A549 using 24300-1-AP

    WB analysis of A549 using 24300-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24300-1-AP (LIPI Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24300-1-AP

    IHC staining of human testis using 24300-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24300-1-AP (LIPI antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 24300-1-AP

    IHC staining of human testis using 24300-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24300-1-AP (LIPI antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inA549 cells, mouse lung tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24300-1-AP is LIPI Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen LIPI fusion protein Ag19024 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lipase, member I
Synonyms Cancer/testis antigen 17, CT17, Lipase member I, lipase, member I, LIPI, LPD lipase, LPDL, mPA PLA1 beta, PRED5
Calculated Molecular Weight 460 aa, 55 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC148691
Gene Symbol LIPI
Gene ID (NCBI) 149998
RRIDAB_2879483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6XZB0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LIPI antibody 24300-1-APDownload protocol
IHC protocol for LIPI antibody 24300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols