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验证数据展示

  • WB analysis using 11496-1-AP

    WB analysis using 11496-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11496-1-AP (LMAN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 11496-1-AP

    IHC staining of mouse testis using 11496-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11496-1-AP (LMAN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMAN2 antibody included
  • IHC staining of mouse testis using 11496-1-AP

    IHC staining of mouse testis using 11496-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11496-1-AP (LMAN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMAN2 antibody included

LMAN2 Polyclonal antibody

LMAN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11496-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

VIP36, Vesicular integral-membrane protein VIP36, Vesicular integral-membrane protein 36, Lectin mannose-binding 2, Glycoprotein GP36b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, rat heart tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LMAN2 Ready-To-Use IHC Kit. LMAN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11496-1-AP targets LMAN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LMAN2 fusion protein Ag1966 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lectin, mannose-binding 2
别名 VIP36, Vesicular integral-membrane protein VIP36, Vesicular integral-membrane protein 36, Lectin mannose-binding 2, Glycoprotein GP36b
计算分子量 356 aa, 40 kDa
观测分子量35-36 kDa
GenBank蛋白编号BC017263
基因名称 LMAN2
Gene ID (NCBI) 10960
RRIDAB_3085375
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12907
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LMAN2 antibody 11496-1-APDownload protocol
IHC protocol for LMAN2 antibody 11496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols