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LMAN2 Polyclonal antibody

LMAN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 11496-1-AP

Print datasheet

Synonyms

VIP36, Vesicular integral-membrane protein VIP36, Vesicular integral-membrane protein 36, Lectin mannose-binding 2, Glycoprotein GP36b

验证数据展示

  • WB analysis using 11496-1-AP

    WB analysis using 11496-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11496-1-AP (LMAN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 11496-1-AP

    IHC staining of mouse testis using 11496-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11496-1-AP (LMAN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMAN2 antibody included
  • IHC staining of mouse testis using 11496-1-AP

    IHC staining of mouse testis using 11496-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11496-1-AP (LMAN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMAN2 antibody included

经过测试的应用

Positive WB detected inHeLa cells, rat heart tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LMAN2 Ready-To-Use IHC Kit. LMAN2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11496-1-AP targets LMAN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LMAN2 fusion protein Ag1966 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lectin, mannose-binding 2
Synonyms VIP36, Vesicular integral-membrane protein VIP36, Vesicular integral-membrane protein 36, Lectin mannose-binding 2, Glycoprotein GP36b
Calculated Molecular Weight 356 aa, 40 kDa
Observed Molecular Weight35-36 kDa
GenBank Accession NumberBC017263
Gene Symbol LMAN2
Gene ID (NCBI) 10960
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12907
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LMAN2 antibody 11496-1-APDownload protocol
IHC protocol for LMAN2 antibody 11496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols