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LMCD1 Monoclonal antibody, PBS Only

LMCD1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A7B3

Cat no : 67834-1-PBS

Print datasheet

Synonyms

Dyxin, LMCD1

验证数据展示

  • WB analysis of U2OS using 67834-1-Ig (same clone as 67834-1-PBS)

    WB analysis of U2OS using 67834-1-Ig (same clone as 67834-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 67834-1-Ig (same clone as 67834-1-PBS)

    IF Staining of MCF-7 using 67834-1-Ig (same clone as 67834-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LMCD1 antibody (67834-1-Ig, Clone: 2A7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

    IHC staining of mouse skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LMCD1 antibody included
  • IHC staining of human colon using 67834-1-Ig (same clone as 67834-1-PBS)

    IHC staining of human colon using 67834-1-Ig (same clone as 67834-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LMCD1 antibody included
Planning an IHC experiment? We recommend our IHCeasy LMCD1 Ready-To-Use IHC Kit. LMCD1 primary antibody included.

产品信息

67834-1-PBS targets LMCD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen LMCD1 fusion protein Ag28331 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name LIM and cysteine-rich domains 1
Synonyms Dyxin, LMCD1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight~40 kDa
GenBank Accession NumberBC000646
Gene Symbol LMCD1
Gene ID (NCBI) 29995
RRIDAB_2918596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NZU5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LIM and cysteine-rich domains-1 (LMCD1) is a member of the LIM protein family, which contains an N-terminal cysteine-rich region, two C-terminal LIM domains and a central PET (Prickle, Espinas, and Testin) domain. LMCD1 has been reported in cardiac tissues and lung acting as a transcriptional repressor for GATA6. The mutations of LMCD1 promote cell migration and tumor metastasis in hepatocellular carcinoma. (PMID: 31501411) It has calculated molecular weight around 41kDa.