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LMF1 Polyclonal antibody

LMF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16812-1-AP

Print datasheet

Synonyms

C16orf26, HMFN1876, JFP11, lipase maturation factor 1, LMF1, TMEM112, TMEM112A, Transmembrane protein 112

验证数据展示

  • WB analysis of SKOV-3 using 16812-1-AP

    WB analysis of SKOV-3 using 16812-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16812-1-AP (LMF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 16812-1-AP

    IHC staining of human liver cancer using 16812-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16812-1-AP (LMF1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 16812-1-AP

    IHC staining of human liver cancer using 16812-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16812-1-AP (LMF1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inSKOV-3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 16812-1-AP is LMF1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen LMF1 fusion protein Ag10349 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lipase maturation factor 1
Synonyms C16orf26, HMFN1876, JFP11, lipase maturation factor 1, LMF1, TMEM112, TMEM112A, Transmembrane protein 112
Calculated Molecular Weight 567 aa, 65 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC010738
Gene Symbol LMF1
Gene ID (NCBI) 64788
RRIDAB_2878320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96S06
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LMF1, also named as C16orf26 and TMEM112, is involved in the maturation of lipoprotein lipase (LPL) and hepatic lipase in endoplasmic reticulum. It is required for maturation and transport of active lipoprotein lipase (LPL) through the secretory pathway.

实验方案

Product Specific Protocols
WB protocol for LMF1 antibody 16812-1-APDownload protocol
IHC protocol for LMF1 antibody 16812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Legal Med

Candidate biomarkers in brown adipose tissue for post-mortem diagnosis of fatal hypothermia

Authors - Miao Zhang
View Article