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LMF2 Polyclonal antibody

LMF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16249-1-AP

Print datasheet

Synonyms

lipase maturation factor 2, LMF2, TMEM112B, TMEM153, Transmembrane protein 112B, Transmembrane protein 153

验证数据展示

  • WB analysis of HeLa using 16249-1-AP

    WB analysis of HeLa using 16249-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16249-1-AP (LMF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 16249-1-AP

    IHC staining of human kidney using 16249-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16249-1-AP (LMF2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 16249-1-AP

    IHC staining of human kidney using 16249-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16249-1-AP (LMF2 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of MCF-7 using 16249-1-AP

    IF Staining of MCF-7 using 16249-1-AP

    Immunofluorescent analysis of MCF-7 cells, using 16249-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHeLa cells, human brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16249-1-AP targets LMF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LMF2 fusion protein Ag9208 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lipase maturation factor 2
Synonyms lipase maturation factor 2, LMF2, TMEM112B, TMEM153, Transmembrane protein 112B, Transmembrane protein 153
Calculated Molecular Weight 682 aa, 77 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC014652
Gene Symbol LMF2
Gene ID (NCBI) 91289
RRIDAB_2136142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BU23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LMF2 antibody 16249-1-APDownload protocol
IHC protocol for LMF2 antibody 16249-1-APDownload protocol
IF protocol for LMF2 antibody 16249-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols