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验证数据展示

  • WB analysis using 29392-1-AP

    WB analysis using 29392-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29392-1-AP (LMO7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 29392-1-AP

    IHC staining of mouse heart using 29392-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29392-1-AP (LMO7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMO7 antibody included
  • IHC staining of mouse heart using 29392-1-AP

    IHC staining of mouse heart using 29392-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29392-1-AP (LMO7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMO7 antibody included
  • IHC staining of mouse skeletal muscle using 29392-1-AP

    IHC staining of mouse skeletal muscle using 29392-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29392-1-AP (LMO7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMO7 antibody included
  • IHC staining of mouse skeletal muscle using 29392-1-AP

    IHC staining of mouse skeletal muscle using 29392-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29392-1-AP (LMO7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LMO7 antibody included

LMO7 Polyclonal antibody

LMO7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29392-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

F box only protein 20, FBX20, FBXO20, KIAA0858, LIM domain 7, LIM domain only protein 7, LMO 7, LMO7, LOMP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, mouse lung tissue
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LMO7 Ready-To-Use IHC Kit. LMO7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29392-1-AP targets LMO7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 LMO7 fusion protein Ag30016 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM domain 7
别名 F box only protein 20, FBX20, FBXO20, KIAA0858, LIM domain 7, LIM domain only protein 7, LMO 7, LMO7, LOMP
计算分子量 193 kDa
观测分子量 140-160 kDa
GenBank蛋白编号NM_005358
基因名称 LMO7
Gene ID (NCBI) 4008
RRIDAB_3086128
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WWI1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LMO7, also known as LOMP, FBX20, belongs to the single Calponin-homology (CH) domain-containing protein family, with a single actin-binding CH domain at the amino terminal end(PMID: 19459066). LMO7 also contains a PDZ domain and a carboxyl-terminal LIM domain, both of which have been described as protein-protein interacting domains(PMID: 15140894). LMO7 encodes three splice variants of calculated molecular weights of 196 kDa (P200), 157 kDa (P150), and 100 kDa (P100)(PMID: 26157009).

实验方案

Product Specific Protocols
WB protocol for LMO7 antibody 29392-1-APDownload protocol
IHC protocol for LMO7 antibody 29392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols