验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14948-1-AP targets LMOD3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, zebrafish, xenopus |
| 免疫原 | LMOD3 fusion protein Ag6758 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leiomodin 3 (fetal) |
| 别名 | Leiomodin 3, leiomodin 3 (fetal), Leiomodin, fetal form, LMOD3 |
| 计算分子量 | 65 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC039202 |
| 基因名称 | LMOD3 |
| Gene ID (NCBI) | 56203 |
| RRID | AB_2136690 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q0VAK6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The gene encoding LMOD3 has not been characterized so far and very limited information of its function has been reported. Nanda et al. found that the expression of mouse LMOD3 mRNA is restricted largely to cardiac and skeletal muscle through RT-PCR analysis (PMID: 22157009). Two isoforms of LMOD3 may exist due to the alternative splicing, whose molecular weights are predicted as 65 kDa and 40 kDa, respectively (Uniprot). This antibody was raised against the N-terminal region of human LMOD3. It detects a double bands around 80 kDa and 65 kDa in heart and skeletal muscle lysates. The reason causing the discrepancy between the predicted and observed molecular weight is not clear.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LMOD3 antibody 14948-1-AP | Download protocol |
| IHC protocol for LMOD3 antibody 14948-1-AP | Download protocol |
| IP protocol for LMOD3 antibody 14948-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Nebulin and Lmod2 are critical for specifying thin-filament length in skeletal muscle. | ||
J Clin Invest Leiomodin-3 dysfunction results in thin filament disorganization and nemaline myopathy. | ||
J Clin Invest Severe myopathy in mice lacking the MEF2/SRF-dependent gene leiomodin-3.
| ||
J Clin Invest Leiomodin-3 dysfunction results in thin filament disorganization and nemaline myopathy. | ||
J Clin Invest KLHL40 deficiency destabilizes thin filament proteins and promotes nemaline myopathy. | ||
Proc Natl Acad Sci U S A HSPB7 is indispensable for heart development by modulating actin filament assembly. |
