验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IF detected in | embryonic kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18278-1-AP targets LMX1B in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | LMX1B fusion protein Ag13096 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | LIM homeobox transcription factor 1, beta |
| 别名 | LIM/homeobox protein 1.2, LIM/homeobox protein LMX1B, LMX 1.2, LMX1.2, LMX1B |
| 计算分子量 | 372 aa, 42 kDa |
| 观测分子量 | 42-45 kDa |
| GenBank蛋白编号 | BC112120 |
| 基因名称 | LMX1B |
| Gene ID (NCBI) | 4010 |
| RRID | AB_11232226 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60663 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LMX1B, LIM homeodomain factor 1b, functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Lmx1b is a continuously expressed, terminal selector-type factor in 5-HT neurons. It is required for maintenance of central serotonergic neurons (PMID: 17151281, PMID: 14602809). It is expressed in brain, testis, skeletal muscle, and pancreatic islets (PMID: 35417751, PMID: 18512225).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LMX1B antibody 18278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons. | ||
Sci Signal Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn. | ||
Neurobiol Dis Maternal Herpesviridae infection during pregnancy alters midbrain dopaminergic signatures in adult offspring. | ||
Biomed Pharmacother LMX1B involved in the radioresistance, proliferation and migration of esophageal cancer cells.
| ||
Methods Mol Biol Imaging Autophagy in hiPSC-Derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease Research. | ||
J Cell Biol ATG8-dependent LMX1B-autophagy crosstalk shapes human midbrain dopaminergic neuronal resilience
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