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AGPHD1 Polyclonal antibody

AGPHD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26070-1-AP

Print datasheet

Synonyms

5-hydroxy-L-lysine kinase, AGPHD1, Hydroxylysine kinase, HYKK

验证数据展示

  • WB analysis of mouse liver using 26070-1-AP

    WB analysis of mouse liver using 26070-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26070-1-AP (LOC123688 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26070-1-AP is AGPHD1 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen AGPHD1 fusion protein Ag23420 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name similar to RIKEN cDNA C630028N24 gene
Synonyms 5-hydroxy-L-lysine kinase, AGPHD1, Hydroxylysine kinase, HYKK
Observed Molecular Weight 27-30 kDa
GenBank Accession NumberBC132753
Gene Symbol AGPHD1/HYKK
Gene ID (NCBI) 123688
RRIDAB_2880361
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA2RU49
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGPHD1 encodes the 5-hydroxy-L-lysine kinase which catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine. It is primarily present in liver and kidneys, suggesting its major role in degrading 5Hyl derived from food. Its presence at lower levels in other tissues indicates that it participates also in the catabolism of 5Hyl derived from local collagen breakdown.

实验方案

Product Specific Protocols
WB protocol for AGPHD1 antibody 26070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols