• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

LOC286135 Polyclonal antibody

LOC286135 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21958-1-AP

Print datasheet

Synonyms

hypothetical LOC286135, LOC286135

验证数据展示

  • WB analysis of mouse brain using 21958-1-AP

    WB analysis of mouse brain using 21958-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21958-1-AP (LOC286135 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 21958-1-AP

    IHC staining of human testis using 21958-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21958-1-AP (LOC286135 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 21958-1-AP

    IHC staining of human testis using 21958-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21958-1-AP (LOC286135 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21958-1-AP targets LOC286135 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen LOC286135 fusion protein Ag16644 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hypothetical LOC286135
Synonyms hypothetical LOC286135, LOC286135
Calculated Molecular Weight 138 aa, 15 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC126344
Gene Symbol LOC286135
Gene ID (NCBI) 286135
RRIDAB_11042462
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LOC286135 antibody 21958-1-APDownload protocol
IHC protocol for LOC286135 antibody 21958-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols