- Featured Product
- KD/KO Validated
LOH12CR1 Polyclonal antibody
LOH12CR1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells |
Positive IHC detected in | human lung cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 4 publications below |
产品信息
17169-1-AP targets LOH12CR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | LOH12CR1 fusion protein Ag10695 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | loss of heterozygosity, 12, chromosomal region 1 |
Synonyms | Myrlysin, Myristoylated lysosomal protein, Loss of heterozygosity 12 chromosomal region 1, LOH1CR12, BORCS5 |
Calculated Molecular Weight | 196 aa, 22 kDa |
Observed Molecular Weight | 17-22 kDa |
GenBank Accession Number | BC013668 |
Gene Symbol | LOH12CR1 |
Gene ID (NCBI) | 118426 |
RRID | AB_2137150 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q969J3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for LOH12CR1 antibody 17169-1-AP | Download protocol |
IHC protocol for LOH12CR1 antibody 17169-1-AP | Download protocol |
IF protocol for LOH12CR1 antibody 17169-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Autophagy HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons. | ||
Cell Rep Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons.
| ||
Cell Genom Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders |