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LONP1 Recombinant antibody, PBS Only (Capture)

LONP1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240460G10

Cat no : 83552-2-PBS

Print datasheet

Synonyms

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53

验证数据展示

  • WB analysis using 83552-2-RR (same clone as 83552-2-PBS)

    WB analysis using 83552-2-RR (same clone as 83552-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83552-2-RR (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83552-2-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83552-2-RR (same clone as 83552-2-PBS)

    IHC staining of human ovary cancer using 83552-2-RR (same clone as 83552-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83552-2-RR (LONP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83552-2-PBS in a different storage buffer formulation.

  • FC experiment of U-251 using 83552-2-RR (same clone as 83552-2-PBS)

    FC experiment of U-251 using 83552-2-RR (same clone as 83552-2-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug LONP1 Recombinant antibody (83552-2-RR, Clone:240460G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83552-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00531-1

    Cytometric bead array standard curve of MP00531-1

    Cytometric bead array standard curve of MP00531-1, LONP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83552-2-PBS. Detection antibody: 83552-1-PBS. Standard: Ag7196. Range: 0.469-60 ng/mL.

  • Affinity and Kinetic Characterization of 83552-2-RR

    Affinity and Kinetic Characterization of 83552-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83552-2-RR against Human LONP1 were performed. The affinity constant is 1.76 nM.

产品信息

83552-2-PBS targets LONP1 as part of a matched antibody pair:

MP00531-1: 83552-2-PBS capture and 83552-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LONP1 fusion protein Ag7196 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lon peptidase 1, mitochondrial
Synonyms Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53
Calculated Molecular Weight 106 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC000235
Gene Symbol LONP1
Gene ID (NCBI) 9361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36776
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then progressively degraded(PMID:15870080). LONP1 has some isoforms with the MW of 106 kDa, 100 kDa and 86 kDa.