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LONP1 Recombinant antibody, PBS Only (Capture)

LONP1 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240460E5

Cat No : 83552-3-PBS

Print datasheet

Synonyms

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53

验证数据展示

  • IF Staining of HepG2 using 83552-3-RR (same clone as 83552-3-PBS)

    IF Staining of HepG2 using 83552-3-RR (same clone as 83552-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (83552-3-RR, Clone: 240460E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83552-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83552-3-RR (same clone as 83552-3-PBS)

    IF Staining of HepG2 using 83552-3-RR (same clone as 83552-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (83552-3-RR, Clone: 240460E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83552-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83552-3-RR (same clone as 83552-3-PBS)

    FC experiment of HeLa using 83552-3-RR (same clone as 83552-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LONP1 Recombinant antibody (83552-3-RR, Clone:240460E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83552-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00531-3

    Cytometric bead array standard curve of MP00531-3

    Cytometric bead array standard curve of MP00531-3, LONP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83552-3-PBS. Detection antibody: 83552-1-PBS. Standard: Ag7196. Range: 0.938-60 ng/mL.

  • Affinity and Kinetic Characterization of 83552-3-RR

    Affinity and Kinetic Characterization of 83552-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83552-3-RR against Human LONP1 were performed. The affinity constant is 1.38 nM.

产品信息

83552-3-PBS targets LONP1 as part of a matched antibody pair:

MP00531-3: 83552-3-PBS capture and 83552-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen LONP1 fusion protein Ag7196 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lon peptidase 1, mitochondrial
Synonyms Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53
Calculated Molecular Weight 106 kDa
GenBank Accession NumberBC000235
Gene Symbol LONP1
Gene ID (NCBI) 9361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36776
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C