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  • Featured Product
  • KD/KO Validated

LONP2 Polyclonal antibody

LONP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 18035-1-AP

Print datasheet

Synonyms

LONPL, LONP, Lon protease-like protein 2, Lon protease homolog 2, peroxisomal, Lon protease 2

验证数据展示

  • IHC staining of human prostate cancer using 18035-1-AP

    IHC staining of human prostate cancer using 18035-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18035-1-AP (LONP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LONP2 antibody included
  • IHC staining of human prostate cancer using 18035-1-AP

    IHC staining of human prostate cancer using 18035-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18035-1-AP (LONP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LONP2 antibody included
  • WB analysis of Jurkat using 18035-1-AP

    WB analysis of Jurkat using 18035-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18035-1-AP (LONP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 18035-1-AP

    IF Staining of HepG2 using 18035-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18035-1-AP (LONP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy LONP2 Ready-To-Use IHC Kit. LONP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18035-1-AP targets LONP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen LONP2 fusion protein Ag12650 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lon peptidase 2, peroxisomal
Synonyms LONPL, LONP, Lon protease-like protein 2, Lon protease homolog 2, peroxisomal, Lon protease 2
Calculated Molecular Weight 852 aa, 95 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC093910
Gene Symbol LONP2
Gene ID (NCBI) 83752
RRIDAB_2878485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WA8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LONP2 antibody 18035-1-APDownload protocol
IHC protocol for LONP2 antibody 18035-1-APDownload protocol
IF protocol for LONP2 antibody 18035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Gene

Role of LONP2 in head and neck squamous cell carcinoma

Authors - Xiaobo Cui
  • KD Validated
View Article